Thermo Scientific Q Exactive Focus

Mass spectrometer with quadrupole precursor ion selection and a high-resolution accurate-mass Orbitrap mass analyser
Appropriate for LC-MS/MS applications (HCD cell)
Resolving power of up to 70,000 (FWHM) at 200 m/z
50 to 3000 m/z
Sub ppm mass accuracy
Rapid polarity switching
Linear dynamic range up to six orders of magnitude
Acquisition possible via Selected Ion Monitoring (SIM), Parallel Reaction Monitoring (PRM), and Data-Independent Acquisition (DIA)

In Stock

Description

Thermo Q Exactive Focus Mass Spectrometer

Benchtop LC-MS/MS system with quadrupole for precursor ion selection, higher-energy collisional dissociation (HCD) cell and Orbitrap high resolution accurate mass analyser. This system is particularly appropriate for the routine analysis of small molecules, and can be used for both targeted and untargeted applications. We are happy to provide Xcalibur software, an LC system and/or rough pump as required. Warranty options are available, please get in touch!

Performance:

Resolving power 70,000 at 200 m/z
Mass range 50 to 3000 m/z
Scan rate up to 12 Hz at resolution setting of 17,500 at 200 m/z*
Mass accuracy Internal: <1 ppm RMS External: <3 ppm RMS
Dynamic range >5000:1
Linear Dynamic >1,000,000 range
Polarity switching One full cycle in <1 sec (one full positive mode scan and one full negative mode scan at a resolution setting of 35,000)
Analog inputs One (1) analog input (0–1 V) One (1) analog (0–10 V)

Operation modes:

Full MS with high-resolution accurate- mass detection
Selected Ion Monitoring (SIM) with high-resolution accurate-mass detection
Parallel Reaction Monitoring (PRM) for improved screening and quantitative confidence
Data-Independent Acquisition (DIA) for unbiased identification and quantitative analysis
All-Ion Fragmentation (AIF) in the HCD collision cell with high-resolution accurate-mass detection
In-source fragmentation of all ions
Positive/negative ion switching on chromatographic timescale
Timed SIM for scheduled data acquisition of the targets of interest

Hardware Specifications:

Ion Source

Removable metal ion transfer capillary provides vent-free maintenance

Ion Optics

RF lens stacked-ring radio frequency ion guide captures and efficiently focuses the ions into a tight beam. Large variable spacing between electrodes allows for better pumping efficiency and improved ruggedness.
Bent flatapole ion guide reduces noise by preventing neutrals and high-velocity clusters from entering the quadrupole

Quadrupole Mass Filter

Thermo Scientific™ HyperQuad™ mass f ilter provides increased ion transmission
Variable precursor ion isolation width selection from 0.4 Da to full mass range HCD Cell C-Trap